Movies

a. Bead movement by fungal kinesin
Purified kinesin-1 from Neurospora crassa moves latex beads along pig brain microtubules (Steinberg and Schliwa, 1995, Mol Biol Cell, 6, 1605). The movie runs at real time, the velocity of this movement is approx 1 micrometer per second.

View the 'Bead movement by fungal kinesin' movie
(509Kb – Opens in a new window)

b. Microtubule gliding over fungal kinesin
Pig brain microtubules slide over glass-attached kinesin-1 from Neurospora crassa (Steinberg and Schliwa, 1995, Mol Biol Cell, 6, 1605). The movie runs at real time, the velocity of this movement is approx 2-3 micrometer per second.

View the 'Microtubule gliding over fungal kinesin' movie
(520Kb – Opens in a new window)

c. Axoneme moving over fungal kinesin
A Chlamydomonas axoneme (central body of the flagellum) slides over glass-attached kinesin-1 from Neurospora crassa (Steinberg and Schliwa, 1995, Mol Biol Cell, 6, 1605). The movie runs at real time, the velocity of this movement is approx 0.7 micrometer per second. Note that the pointed end represents the minus-ends of the microtubules in the axoneme. Neurospora kinesin moves to plus-ends and pushes the pointed end of the axoneme forward.

View the 'Axoneme moving over fungal kinesin' movie
(400Kb – Opens in a new window)

a. Neurospora_Spitzenkörper
N. crassa hypha that grow through low melt agarose. Membranous vesicles accumulate in the hyphal tip. A pseudo coloured detail is given left. Elapsed time is given in minutes:seconds.

View the 'Neurospora_Spitzenkörper' movie
(1Mb – Opens in a new window)

b. Neurospora_nuclear migration
Nuclear migration in a wall-less N. crassa mutant, defective in β-D-glucan synthase (fz;sg;os-1; FGSC 1118), in phosphate buffered saline. The movie runs at real time, the elapsed time is given in minutes:seconds.

View the 'Neurospora_nuclear migration' movie
(952Kb – Opens in a new window)

c. Neurospora_motility of mitochondria
Motility of mitochondria in a wall-less N. crassa mutant, defective in β-D-glucan synthase (fz;sg;os-1; FGSC 1118), in phosphate buffered saline. The movie runs at real time, the elapsed time is given in minutes:seconds.

View the 'Neurospora_motility of mitochondria' movie
(1.4Mb – Opens in a new window)

a. Neurospora_crawling pseudopdia
Motility of a wall-less N. crassa mutant cell attached to a protamine-sulfate coated cover slip. The movie runs at real time, the elapsed time is given in minutes:seconds.

View the 'Neurospora_crawling pseudopdia' movie
(796Kb – Opens in a new window)

b. Neurospora_waving SLIME
Motility of a wall-less N. crassa mutant, defective in β-D-glucan synthase (fz;sg;os-1; FGSC 1118), in phosphate buffered saline. The movie runs at real time, the elapsed time is given in minutes:seconds.

View the 'Neurospora_waving SLIME' movie
(1.1Mb – Opens in a new window)

a. Ustilago_Microtubules in yeast-like sporidia
3D-reconstruction of GFP-alpha tubulin labelled microtubules.

View the 'Ustilago_Microtubules in yeast-like sporidia' movie
(660Kb – Opens in a new window)

b. Ustilago_MT plus ends
Orientation of microtubules in a hypha of U. maydis that express a functional fusion protein of the EB1 homologue Peb1 and RFP and GFP-αtubulin αtubulin (from Schuchardt et al 2005, Mol Biol Cell., 16, 5191) Elapsed time is given in seconds.

View the 'Ustilago_MT plus ends' movie
(68Kb – Opens in a new window)

c. Ustilago_microtubule motility
Organisation and motility of microtubules in hyphae of U. maydis. Microtubules are labelled by an ectopically integrated GFP-αtubulin gene. Note that this motility is driven by dynein Elapsed time is given in seconds.

View the 'Ustilago_microtubule motility' movie
(236Kb – Opens in a new window)

a. Ustilago_3GFP-Myosin-5
GFP-labelled Myosin-5 expressed in a myo5 null mutant of U. maydis. Boxes indicate the regions for which magnifications are given. Note that individual signals move over long distances within the hypha. Elapsed time is given in seconds.

View the 'Ustilago_3GFP-Myosin-5' movie
(256Kb – Opens in a new window)

a. Ustilago_Yup1 on early endosomes
Motility of GFP-tagged tSNARE Yup1 (Wedlich-Söldner et al. 2000, EMBO J. 19, 1974). This motility is powered by kinesin-3 (see movie 7). Elapsed time is given in minutes:seconds.

View the 'Ustilago_Yup1 on early endosomes' movie
(188Kb – Opens in a new window)

b. Ustilago_EE motility along microtubules
Motility of early endosomes in a hypha, visualized by the expression of the putative tSNARE Yup1 fused to GFP, along microtubules that are labelled by RFP-αtubulin. Elapsed time is given in seconds.

View the 'Ustilago_EE motility along microtubules' movie
(172Kb – Opens in a new window)

c. Ustilago_ Co-localizing Rab5a and Yup1
The early endosome marker Rab5, tagged with GFP, and the tSNARE Yup1, labelled with mRFP, co-localize on moving endosomes (Fuchs et al 2006, Plant Cell, 18, 2066). Note that Yup1 is also found on vacuolar membranes. Elapsed time is given in seconds:milliseconds.

View the 'Ustilago_ Co-localizing Rab5a and Yup1' movie
(92Kb – Opens in a new window)

d. Ustilago_Motility of kinesin3-GFP
Motility of a functional kinesin-3-GFP fusion protein, expressed in a kin3 null mutant. Boxes indicate the regions for which magnifications are given. Elapsed time is given in seconds.

View the 'Ustilago_Motility of kinesin3-GFP' movie
(368Kb – Opens in a new window)

e. Movement of the GTPase Rab5a along microtubules in hyphal cell of U. maydis
Rab5a is N-terminally tagged by GFP, microtubules are labeled by mCherry. Time is given in seconds:milliseconds. The data were acquired using Laser-Excitation-Epi-Fluorescence (LEEM).

View the 'Movement of the GTPase Rab5a along microtubules in hyphal cell of U. maydis' movie
(247Kb – Opens in a new window)

a. Ustilago_Dynein appearance in anaphase A
Endogenous dynein, tagged with mRFP appears at astral microtubules at the onset of anaphase B from where it pulls on the spindle an separates the chromosomes(Fink et al 2006, EMBO J. 25, 4897). Elapsed time is given in minutes:seconds.

View the 'Ustilago_Dynein appearance in anaphase A' movie
(52Kb – Opens in a new window)

b. Ustilago_Dynein in anaphase B
Endogenous dynein, tagged with mRFP localizes to the spindle pole bodies and the plus-ends ends of astral microtubules (Fink et al 2006, EMBO J. 25, 4897). Elapsed time is given in seconds:milliseconds.

View the 'Ustilago_Dynein in anaphase B' movie
(72Kb – Opens in a new window)

c. Ustilago_Nuclear porins in open mitosis
The nuclear pore complex disassembles in mitosis. A fraction of the nuclear porin Nup107 (endogenously tagged with GFP) migrates to the DNA (arrowhead), whereas the membrane-bound Pom152 homologue (mRFP) disperses in the ER (Theisen et al 2008, Mol. Biol Cell, 19, 1230). Elapsed time is given in minutes:seconds.

View the 'Ustilago_Nuclear porins in open mitosis' movie
(56Kb – Opens in a new window)

d. Ustilago_ Reformation of NE in anaphase
The chromosomes (labelled with Histone 4-RFP) get separated in anaphase. The elongating spindle collects tubules of the endoplasmic reticulum (labelled with GFP-HDEL) are collected that appear to participate in reformation of the nuclear envelope. Elapsed time is given in minutes:seconds.

View the 'Ustilago_ Reformation of NE in anaphase' movie
(68Kb – Opens in a new window)

e. 3D-reconstruction of Nup107-containg nuclear pores in interphase
Maxium reconstruction from a 3D-stack after deconvolution. The data were aquired using Laser-Excitation-Epi-Fluorescence (LEEM).

View the '3D-reconstruction of nuclear pores' movie
(123Kb – Opens in a new window)

a. Endoplasmic retikulum and microtubules
ER is visualized with GFP-SKL that is targeted to the ER and mCherry alpha-tubulin, which labels microtubules. Time is given in seconds:milliseconds. The data were acquired using Laser-Excitation-Epi-Fluorescence (LEEM).

View the 'Endoplasmic retikulum and microtubules' movie
(255Kb – Opens in a new window)

b. Motility of the endoplasmic retikulum along microtubules
ER is visualized with GFP-SKL that is targeted to the ER and mCherry alpha-tubulin, which labels microtubules. Time is given in seconds:milliseconds. The data were acquired using Laser-Excitation-Epi-Fluorescence (LEEM). Note that this motility depends on dynein (Wedlich-Soeldner et al. 2002, MBC, 13, 965).

View the 'Motility of the endoplasmic retikulum along microtubules' movie
(253Kb – Opens in a new window)